CD90 antibody (CF®594)

Catalog No. ABIN6166549

The Mouse Monoclonal anti-CD90 antibody has been validated for FACS and IF. It is suitable to detect CD90 in samples from Human and Cow.

Quick Overview for CD90 antibody (CF®594) (ABIN6166549)

Target: CD90 (THY1)

Reactivity: Human, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD90 antibody is conjugated to CF®594

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: AF-9

Product Details anti-CD90 Antibody (CF®594)

Purpose: Mouse Monoclonal anti-CD90/Thy1 (AF-9), CF594 Conjugate

Characteristics: This antibody recognizes a protein of 18-35 kDa, identified as CD90 (also known as Thy1). CD90 is a member of the immunoglobulin superfamily. It may contribute to inhibition of proliferation/differentiation of hematopoietic stem cells and neuron memory formation in the CNS. It consists of a single Ig domain (112 amino acids, 25-35 kDa) inserted into the cell membrane via a GPI anchor. Expressed by hematopoietic stem cells and neurons in all species studied. Its highly expressed in connective tissue and various fibroblast and stromal cell lines, expressed on all thymocytes and peripheral T cells in mice, but in humans expressed only on small % fetal thymocytes, 10-40 % of CD34 cells in bone marrow, and <1 % of CD3 CD4 lymphocytes in peripheral circulation. It is also expressed by human lymph node HEV endothelium but not other endothelia. Lastly, it is expressed by a limited number of lymphoblastoid and leukemic cell lines. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the < a href="https://biotium.com/wp-content/uploads/2013/07/CF-dye-selection-guide.pdf" target="_blank" rel="noopener">CF® Dye Selection Guide for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human T-lymphoma cells

Isotype: IgG1 kappa

Application Details

Application Notes: Immunofluorescence 0.5-1 μg/mL

• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

IMR-32, CCRF-CEM or MOLT-4 cells. Human uterus.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for CD90

Target: CD90 (THY1)

Alternative Name: CD90/Thy1

Background: CD54, Cell surface glycoprotein P3.58, Human rhinovirus receptor, ICAM-1, Intercellular adhesion molecule 1, Ly 47, Major group rhinovirus receptor, MALA2, MyD10, Surface antigen of activated B cells

Molecular Weight: 18-35 kDa

Gene ID: 7070, 644697

UniProt: P04216

Pathways: Cell-Cell Junction Organization