Podoplanin antibody (AA 24-126) (CF®594)

Catalog No. ABIN6167005

The Mouse Monoclonal anti-Podoplanin antibody has been validated for WB, IHC, FACS and IF. It is suitable to detect Podoplanin in samples from Human.

Quick Overview for Podoplanin antibody (AA 24-126) (CF®594) (ABIN6167005)

Target: Podoplanin (PDPN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Podoplanin antibody is conjugated to CF®594

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: PDPN-1433

Product Details anti-Podoplanin Antibody (CF®594)

Binding Specificity: AA 24-126

Purpose: Mouse Monoclonal anti-Podoplanin (PDPN/1433), CF594 Conjugate

Characteristics: This antibody recognizes a muco-protein of 38-43 kDa, which is identified Podoplanin (PDPN). It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endothelium as well as lymphoangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Podoplanin (PDPN) protein fragment (aa24-126) (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL Western blotting 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

HeLa Cells. Angiosarcoma or Mesothelioma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Podoplanin

Target: Podoplanin (PDPN)

Alternative Name: Podoplanin

Molecular Weight: 38-43 kDa

Gene ID: 10630, 468675

UniProt: Q86YL7

Pathways: Dicarboxylic Acid Transport