S100A1 antibody (Biotin)

Catalog No. ABIN6167352

This Mouse Monoclonal antibody specifically detects S100A1 in IHC and IF. It exhibits reactivity toward Human.

Quick Overview for S100A1 antibody (Biotin) (ABIN6167352)

Target: S100A1 (S100 Calcium Binding Protein A1 (S100A1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A1 antibody is conjugated to Biotin

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: S1-61

Product Details anti-S100A1 Antibody (Biotin)

Purpose: Mouse Monoclonal anti-S100A (S1/61), Biotin Conjugate

Characteristics: S100A and S100B proteins are two members of the S100 family of calcium binding proteins. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the alpha-chain (i.e. in S-100A and S-100B) but not on the beta-chain of S-100 (i.e. in S-100B). This antibody can be used to localize S-100A in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, epithelial and myoepithelial cells of the breast, salivary and sweat glands. Neoplasms derived from these cells also express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified human S-100 protein conjugated to methylated BSA

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology (formalin) 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

A431 or HT-29 Cells. Brain or Melanoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for S100A1

Target: S100A1 (S100 Calcium Binding Protein A1 (S100A1))

Alternative Name: S100A

Background: Bpb, NEF, S100-A1, S100 Alpha Chain, S100 Beta Chain, S100 Calcium Binding Protein A1, S100 Calcium Binding Protein B, S100 Calcium Binding Protein Beta Neural

Molecular Weight: 10-12 kDa

Gene ID: 6271, 515715

UniProt: P23297

Pathways: Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades, S100 Proteins