s100a4 antibody (CF®640R)

Catalog No. ABIN6167365

The Mouse Monoclonal anti-s100a4 antibody has been validated for WB, IHC, IF and FACS. It is suitable to detect s100a4 in samples from Human and Mouse.

Quick Overview for s100a4 antibody (CF®640R) (ABIN6167365)

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This s100a4 antibody is conjugated to CF®640R

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: S100A4-1481

Product Details anti-s100a4 Antibody (CF®640R)

Purpose: Mouse Monoclonal anti-S100A4 (S100A4/1481), CF640R Conjugate

Characteristics: S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, ?6?4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human S100A4 protein

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology (formalin) 0.25-0.5 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HeLa, A549 or A375 Cells. Placenta

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for s100a4

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Alternative Name: S100A4

Molecular Weight: 10-12 kDa

Gene ID: 6275, 654444

UniProt: P26447

Pathways: S100 Proteins