VLDLR antibody (C-Term) (CF®405S)

Catalog No. ABIN6168159

This Mouse Monoclonal antibody specifically detects VLDLR in WB, IHC, FACS, IF and ISt. It exhibits reactivity toward Human and Rat.

Quick Overview for VLDLR antibody (C-Term) (CF®405S) (ABIN6168159)

Target: VLDLR (Very Low Density Lipoprotein Receptor (VLDLR))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This VLDLR antibody is conjugated to CF®405S

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunostaining (ISt)

Clone: VLDLR-1337

Product Details anti-VLDLR Antibody (CF®405S)

Binding Specificity: C-Term

Purpose: Mouse Monoclonal anti-VLDL Receptor (VLDLR/1337), CF405S Conjugate

Characteristics: VLDLR (very low density lipoprotein receptor) is a member of the LDL receptor gene family, which includes LDL receptor, LRP, megalin, VLDLR and ApoER2. The LDL receptor family is characterized by a cluster of cysteine-rich class A repeats, epidermal growth factor (EGF)-like repeats, YWTD repeats and an O-linked sugar domain. VLDLR associates with RAP (receptor associated protein) during receptor folding, and RAP facilitates the secretion of the extracellular region of VLDLR. VLDLR is thought to mediate the interaction of extracellular Reelin and cytosolic mDab1 (mammalian disabled protein), which activates a tyrosine kinase. This pathway regulates the migration of neurons along the radial glial fiber network during brain development. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human VLDLR fragment from c-terminal (exact sequence is proprietary)

Isotype: IgG1

Application Details

Application Notes: Immunohistology (formalin) 1-2 μg/mL

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HeLa or U-251 cells. Pancreas, Cerebellum, Heart or Skeletal Muscle.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for VLDLR

Target: VLDLR (Very Low Density Lipoprotein Receptor (VLDLR))

Alternative Name: VLDL Receptor

Background: Very low-density lipoprotein receptor, VLDL receptor, VLDL-R, VLDLR

Molecular Weight: 143-161 kDa

Gene ID: 7436

Pathways: Cellular Response to Molecule of Bacterial Origin