SHBG antibody (CF®647)

Catalog No. ABIN6170468

This anti-SHBG antibody is a Mouse Monoclonal antibody detecting SHBG in FACS, IF and IHC (f). Suitable for Human.

Quick Overview for SHBG antibody (CF®647) (ABIN6170468)

Target: SHBG (Sex Hormone Binding Globulin (SHBG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SHBG antibody is conjugated to CF®647

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: SHBG-245

Product Details anti-SHBG Antibody (CF®647)

Purpose: Mouse Monoclonal anti-SHBG (Sex Hormone-Binding-Globulin) (SHBG/245), CF647 Conjugate

Characteristics: This antibody recognizes a protein of 45 kDa, identified as SHBG. It functions as an androgen transport protein, but may also be involved in receptor-mediated processes. Each dimer binds one molecule of steroid. It is specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. SHBG regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration. In testis, it is synthesized by the Sertoli cells, secreted into the lumen of the seminiferous tubule and transported to the epididymis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human SHBG protein

Isotype: IgG1

Application Details

Application Notes: Immunohistology formalin-fixed 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

Liver, Placenta or Testis

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for SHBG

Target: SHBG (Sex Hormone Binding Globulin (SHBG))

Alternative Name: SHBG (Sex Hormone-Binding-Globulin)

Molecular Weight: 45 kDa

Gene ID: 6462, 632235

UniProt: P04278

Pathways: Hormone Transport