Cytokeratin 19 antibody (Biotin)

Catalog No. ABIN6172085

This anti-Cytokeratin 19 antibody is a Mouse Monoclonal antibody detecting Cytokeratin 19 in IF, FACS and IHC (f). Suitable for Human and Mouse.

Quick Overview for Cytokeratin 19 antibody (Biotin) (ABIN6172085)

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 19 antibody is conjugated to Biotin

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: BA17

Product Details anti-Cytokeratin 19 Antibody (Biotin)

Purpose: Mouse Monoclonal anti-Cytokeratin 19 (BA17), Biotin Conjugate

Characteristics: This Ab reacts with the rod domain of human cytokeratin 19 (CK19), a polypeptide of 40 kDa. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50 % -60 % of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human mammary epithelial organoids.

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

MCF-7, HeLa cells, Breast Cancer

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Cytokeratin 19

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Alternative Name: Cytokeratin 19

Background: K19, k1cs, Keratin 19 Keratin Type i 40kD, krt19

Molecular Weight: 40 kDa

Gene ID: 3880, 654568

UniProt: P08727