Keratin 10 antibody (CF®647)

Catalog No. ABIN6172254

This Mouse Monoclonal antibody specifically detects Keratin 10 in IHC, FACS and IF. It exhibits reactivity toward Human, Dog and Cat.

Quick Overview for Keratin 10 antibody (CF®647) (ABIN6172254)

Target: Keratin 10 (KRT10)

Reactivity: Human, Dog, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 10 antibody is conjugated to CF®647

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: DE-K10

Product Details anti-Keratin 10 Antibody (CF®647)

Purpose: Mouse Monoclonal anti-Cytokeratin 10 (DE-K10), CF647 Conjugate

Characteristics: This MAb recognizes a protein of 56.5 kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation, it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Cytoskeletal preparation extracted from human ectocervical epithelium

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry 0.5-1 μg/million cells in 0.1 mL

• Immunohistology (formalin) 0.1-0.2 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

A431, HeLa, MCF7 cells. Esophagus or tonsil.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Keratin 10

Target: Keratin 10 (KRT10)

Alternative Name: Cytokeratin 10

Background: BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10

Molecular Weight: 56.5 kDa

Gene ID: 3858, 99936

UniProt: P13645