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KRT15 antibody (C-Term) (CF®640R)

This anti-KRT15 antibody is a Mouse Monoclonal antibody detecting KRT15 in WB, FACS, IF and IHC (fro). Suitable for Human, Rat and Cow.
Catalog No. ABIN6172478

Quick Overview for KRT15 antibody (C-Term) (CF®640R) (ABIN6172478)

Target

See all KRT15 Antibodies
KRT15 (Keratin 15 (KRT15))

Reactivity

  • 154
  • 47
  • 27
  • 27
  • 2
Human, Rat, Cow

Host

  • 88
  • 71
  • 2
Mouse

Clonality

  • 95
  • 66
Monoclonal

Conjugate

  • 64
  • 19
  • 7
  • 6
  • 6
  • 6
  • 6
  • 6
  • 6
  • 5
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This KRT15 antibody is conjugated to CF®640R

Application

  • 91
  • 91
  • 62
  • 52
  • 42
  • 19
  • 16
  • 15
  • 14
  • 14
  • 13
  • 10
  • 6
  • 5
  • 2
  • 1
Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone

LHK15
  • Binding Specificity

    • 18
    • 15
    • 7
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Purpose

    Mouse Monoclonal anti-Cytokeratin 15 (LHK15), CF640R Conjugate

    Characteristics

    Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratin 15 is a type I keratin which is expressed only in basal keratinocytes in stratified epithelia and does not appear to have a natural type II expression partner. Keratin 15 is down regulated in activated keratinocytes. Cytokeratin 15 is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for diagnosis between basal cell carcinoma (BCC) and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceous. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a diagnosis difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    Immunogen

    A 17 amino acids synthetic peptide from the C- terminal of Human Cytokeratin 15 (exact sequence is proprietary)

    Isotype

    IgG2a kappa
  • Application Notes

    Immunohistology (frozen or formalin) 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Western blotting 0.5-1 μg/mL
    • Optimal dilution for a specific application should be determined by user

    Comment

    A431 cells. Skin or Colon

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    100 μg/mL

    Buffer

    PBS/0.1 % BSA/0.05 % azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Protect from light
  • Target

    KRT15 (Keratin 15 (KRT15))

    Alternative Name

    Cytokeratin 15

    Background

    CK15, Cytokeratin 15, K1CO, Ka15, Keratin 15 basic, Keratin 15 beta, Keratin complex 1 acidic gene 15, Keratin type I cytoskeletal 15, KRT15, KRTB, KRTL15, Type I cytoskeletal 15, Type I keratin Ka15

    Molecular Weight

    52 kDa

    Gene ID

    3866, 654570

    UniProt

    P19012
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