FLI1 antibody (AA 107-261)

Catalog No. ABIN6172663

The Mouse Monoclonal anti-FLI1 antibody has been validated for WB, IF and FACS. It is suitable to detect FLI1 in samples from Human.

Quick Overview for FLI1 antibody (AA 107-261) (ABIN6172663)

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FLI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: FLI1-1312

Product Details anti-FLI1 Antibody

Binding Specificity: AA 107-261

Purpose: Mouse Monoclonal anti-FLI1 (FLI1/1312), Purified, BSA-free

Characteristics: This antibody recognizes a protein of 51 kDa, which is identified as FLI1. This protein, a member of the ETS family of DNA binding transcription factors, is involved in cellular proliferation and tumorigenesis. Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus.Members of the Ets gene family share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. Approximately 90 % of Ewing s Sarcoma (EWS)/Primitive Neuroectodermal Tumors (PNET) have a specific translocation, t(11,22)(q24,q12), which results in fusion of EWS to Fli-1, and production of an EWS-Fli-1 fusion protein. Among normal tissues only endothelial cells and small lymphocytes express Fli-1. This protein is expressed in majority of vascular tumors including angiosarcomas, hemangioendotheliomas, hemangiomas, and Kaposi s Sarcomas. High sensitivity and specificity of Fli-1 equals to or exceeds that of the established vascular markers like CD31, CD34, and Factor VIII. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogen: Recombinant fragment (aa 107-261) of human FLI1 protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: Western blotting 1-2 μg/mL

• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

THP1 and Raji cells. Tonsil, Ewing s sarcoma (EWS) and Angiosarcoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Preservative: Azide free

Target Details for FLI1

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Alternative Name: FLI1

Molecular Weight: 51 kDa

Gene ID: 2313, 504281

UniProt: Q01543