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Keratin 5/8 antibody (KRT5/8) (CF®488A) Primary Antibody

KRT5/8 Reactivity: Human, Mouse, Rat, Cow, Dog, Ferret, Hamster, Pig, Sheep FACS, IF, IHC (f), WB Host: Mouse Monoclonal C-50 CF®488A
Catalog No. ABIN6174472
$270.77
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  • Target See all Keratin 5/8 (KRT5/8) Antibodies
    Keratin 5/8 (KRT5/8)
    Reactivity
    • 34
    • 27
    • 25
    • 21
    • 21
    • 19
    • 19
    • 17
    • 15
    • 5
    • 4
    • 2
    • 2
    • 2
    Human, Mouse, Rat, Cow, Dog, Ferret, Hamster, Pig, Sheep
    Host
    • 34
    Mouse
    Clonality
    • 34
    Monoclonal
    Conjugate
    • 13
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Keratin 5/8 antibody is conjugated to CF®488A
    Application
    • 26
    • 23
    • 18
    • 18
    • 13
    • 10
    • 8
    • 6
    • 4
    • 2
    • 2
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
    Purpose
    Mouse Monoclonal anti-Cytokeratin 5/8 (C-50), CF488A Conjugate
    Characteristics
    This antibody recognizes keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Cytoskeletal preparation from HeLa cells
    Clone
    C-50
    Isotype
    IgG1 kappa
  • Application Notes
    Immunohistology formalin-fixed 0.5-1.0 μg/mL
    • For staining of formalin-fixed tissues, digest sections with trypsin at 1 mg/mL PBS, 15 min at RT
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 0.5-1.0 μg/mL
    • Western blotting 0.5-1.0 μg/mL
    • Does not react with chicken , or Xenopus
    • Optimal dilution for a specific application should be determined by user
    Comment

    HepG2 or A431 cells. Skin or Squamous cell carcinoma

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect from light
  • Target
    Keratin 5/8 (KRT5/8)
    Alternative Name
    Cytokeratin 5/8 (KRT5/8 Products)
    Molecular Weight
    58 kDa (CK5), 52.5 kDa (CK8)
    Gene ID
    3852, 3856
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