Keratin 5/8 antibody

Catalog No. ABIN6941336

The Mouse Monoclonal anti-Keratin 5/8 antibody is suitable to detect Keratin 5/8 in samples from Human, Mouse, Rat, Pig, Dog, Hamster, Cow, Sheep and Ferret. It has been validated for WB, FACS, IHC and IF.

Quick Overview for Keratin 5/8 antibody (ABIN6941336)

Target: Keratin 5/8 (KRT5/8)

Reactivity: Human, Mouse, Rat, Pig, Dog, Hamster, Cow, Sheep, Ferret

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 5/8 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: C-50

Product Details anti-Keratin 5/8 Antibody

Purpose: Cytokeratin 5/8 Antibody

Specificity: It reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.

No Cross-Reactivity: Chicken, Xenopus laevis

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Cytoskeletal preparation from HeLa cells

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: HepG2 or A431 cells. Skin or Squamous cell carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(For staining of formalin-fixed tissues, digest sections with trypsin at 1 mg/mL PBS, 15 min at RT)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2 to 8°C. Antibody is stable for 24 months.

Expiry Date: 24 months

Target Details for Keratin 5/8

Target: Keratin 5/8 (KRT5/8)

Alternative Name: KRT5 & KRT8

Background:

CK5, DDD, EBS2, Epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne Types, K5, Keratin complex 2, basic, Gene 5, Keratin Type II Cytoskeletal 5, Keratin-5, KRT5, KRT5A, Type II Cytoskeletal 5, Type-II Keratin Kb5. CK8, CARD2, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8

HepG2 or A431 cells. Skin or Squamous cell carcinoma.

Molecular Weight: 58kDa (CK5), 52.5kDa (CK8)

Gene ID: 3852, 3856, 433845

UniProt: P13647, P05787