Keratin 5/8 antibody

Catalog No. ABIN3025013

Product Details anti-Keratin 5/8 Antibody

Target: Keratin 5/8 (KRT5/8)

Reactivity: Human, Mouse, Pig, Cow, Rat, Dog, Hamster, Sheep, Ferret

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 5/8 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

No Cross-Reactivity: Chicken, Xenopus laevis

Characteristics: It reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.

Purification: Protein G affinity chromatography

Immunogen: A cytoskeletal preparation from HeLa cells was used as the immunogen for the Cytokeratin 5/8 antibody.

Clone: SPM268

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the Cytokeratin 5/8 antibody should be determined by the researcher.

1. For staining of formalin-fixed tissues, digest sections with Trypsin at 1 mg/mL PBS, 15 min at RT
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Cytokeratin 5/8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Keratin 5/8

Target: Keratin 5/8 (KRT5/8)

Alternative Name: Cytokeratin 5/8 (KRT5/8 Products)

Background: It reacts with keratin 5 (58  kDa) and keratin 8 (52.5  kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.