Keratin 5/8 antibody (AA 353-367) (Biotin)

Catalog No. ABIN344035

Product Details anti-Keratin 5/8 Antibody (Biotin)

Target: Keratin 5/8 (KRT5/8)

Binding Specificity: AA 353-367

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 5/8 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA

Specificity: Reactivities on Cultured MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650. Cell lines (tested so far) SSC-12, bovine BMGE+H, BMGE-H, MDBK. Represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. Specifically reacts with cytokeratins 5 and 8 present in nearly all epithelia. Polypeptide reacting: Mr 52500, Mr 58000 cytokeratins (type II cytokeratins nos. 5+8) of human epithelial cells. Epitope has been mapped to aa353-367 on alpha helical rod domain. Species cross-reactivity: Human, bovine, murine, amphibia, porcine, hydra.

Purification: Protein A purified

Immunogen: Human cytokeratin 8, purified from SDS PAGE.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo, IHC, IHC-Fr, IHC-P

Usage: Suitable for use in ELISA, FACS and Immunohistochemistry (frozen and paraffin-embedded tissue and cytological material, Pretreatment With paraffin embedded sections, protease pretreatment is required prior to antibody application). Immunohistochemistry: 1:10-1:20, Incubation Time 1hr at RT, extended with paraffin sections (overnight at 2-8?C). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Target Details for Keratin 5/8

Target: Keratin 5/8 (KRT5/8)

Alternative Name: Cytokeratin 5+8 (KRT5/8 Products)

Background: This antibody detects: Cytokeratin 5+8