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BCL2L1 antibody (AA 3-14) (CF®488A)

This anti-BCL2L1 antibody is a Mouse Monoclonal antibody detecting BCL2L1 in WB, IF, FACS and IHC (f). Suitable for Human, Mouse, Rat and Pig.
Catalog No. ABIN6175460

Quick Overview for BCL2L1 antibody (AA 3-14) (CF®488A) (ABIN6175460)

Target

See all BCL2L1 Antibodies
BCL2L1 (BCL2-Like 1 (BCL2L1))

Reactivity

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Human, Mouse, Rat, Pig

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This BCL2L1 antibody is conjugated to CF®488A

Application

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Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone

BX006 2H12
  • Binding Specificity

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    AA 3-14

    Purpose

    Mouse Monoclonal anti-bcl-x (BX006 + 2H12), CF488A Conjugate

    Characteristics

    This antibody recognizes a protein of 27 kDa, identified as the Bcl-X protein. This MAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This MAb reacts with both Bcl-XS and Bcl-XL proteins. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    Immunogen

    A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein (BX006 & 2H12)

    Isotype

    IgG
  • Application Notes

    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Western blotting 0.5-1 μg/mL
    • Optimal dilution for a specific application should be determined by user

    Comment

    Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin s lymphoma.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    100 μg/mL

    Buffer

    PBS/0.1 % BSA/0.05 % azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Protect from light
  • Target

    BCL2L1 (BCL2-Like 1 (BCL2L1))

    Alternative Name

    bcl-x

    Background

    Apoptosis regulator Bcl-X, B cell lymphoma 2 like, BCL XL/S, Bcl-2-like protein 1, Bcl2-L-1, BCL2L, Bcl2l1, BCLX, BclXL, BclXs, Protein phosphatase 1 regulatory subunit 52 (PPP1R52)

    Molecular Weight

    27 kDa

    Gene ID

    598, 516966

    UniProt

    Q07817

    Pathways

    Apoptosis, Negative Regulation of intrinsic apoptotic Signaling
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