CD20 antibody (CF®647)

Catalog No. ABIN6176206

The Mouse Monoclonal anti-CD20 antibody has been validated for FACS, IF and Func. It is suitable to detect CD20 in samples from Human.

Quick Overview for CD20 antibody (CF®647) (ABIN6176206)

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD20 antibody is conjugated to CF®647

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Clone: 93-1B3

Product Details anti-CD20 Antibody (CF®647)

Purpose: Mouse Monoclonal anti-CD20 (93-1B3), CF647 Conjugate

Characteristics: This antibody recognizes a protein of 30-33 kDa, which is identified as CD20 (Workshop V, Code CD20.4). It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. The protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B-cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as calcium channel involved in B cell activation and cell cycle progression. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Stimulated human leukocytes

Isotype: IgG1 kappa

Application Details

Application Notes: Immunofluorescence 0.5-1 μg/mL

• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Functional Studies order Ab without BSA & azide)
• Optimal dilution for a specific application should be determined by user

Comment:

Daudi, Raji, and U266, and human lymphocytes. Lymph nodes and tonsils.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for CD20

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Alternative Name: CD20

Molecular Weight: 33-37 kDa

Gene ID: 931, 712553

UniProt: P11836