CD3 epsilon antibody

Catalog No. ABIN6176615

The Mouse Monoclonal anti-CD3 epsilon antibody (Clone CRIS-7) (ABIN6176615) specifically detects CD3 epsilon in FACS, IF, Act and ProA. The antibody is reactive with Human and Monkey samples.

Quick Overview for CD3 epsilon antibody (ABIN6176615)

Target: CD3 epsilon (CD3E)

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Activation (Act), Proliferation Assay (ProA)

Clone: CRIS-7

Product Details anti-CD3 epsilon Antibody

Purpose: Mouse Monoclonal anti-CD3e (CRIS-7), Purified, BSA-free

Characteristics: This antibody recognizes the epsilon-chain of CD3 (Workshop V, Code: CD03.09), which consists of five different polypeptide chains (designated as γ, δ, ε, ζ, and η) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogen: Stimulated human leukocytes

Isotype: IgG2a kappa

Application Details

Application Notes: Flow Cytometry 0.5-1 μg/million cells/0.1 mL

• Immunofluorescence 1-2 μg/mL
• Induce T cell activation and proliferation
• Optimal dilution for a specific application should be determined by user

Comment:

Jurkat cells. Tonsil and lymph node

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Preservative: Azide free

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3e

Molecular Weight: 20 kDa

Gene ID: 916, 3003

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway