CD55 antibody (CF®568)

Catalog No. ABIN6177466

The Mouse Monoclonal anti-CD55 antibody has been validated for FACS, IF and IHC (fro). It is suitable to detect CD55 in samples from Human.

Quick Overview for CD55 antibody (CF®568) (ABIN6177466)

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD55 antibody is conjugated to CF®568

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 143-30

Product Details anti-CD55 Antibody (CF®568)

Purpose: Mouse Monoclonal anti-CD55 (DAF) (143-30), CF568 Conjugate

Characteristics: This antibody recognizes a single chain glycoprotein of 70 kDa, identified as CD55 (also known as decay accelerating factor, DAF). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. The MAb 143-30 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH). Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: PHA stimulated human PBL

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology (frozen) 0.5-1 μg/mL

• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

Jurkat, HUT-78, K562, YT, U937, MG63, and human lymphocytes. Human lymph nodes and tonsils.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for CD55

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Alternative Name: CD55 (DAF)

Molecular Weight: 70 kDa

Gene ID: 1604, 126517

UniProt: P08174

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity