GJB1 antibody (CF®488A)

Catalog No. ABIN6178500

This Rat Monoclonal antibody specifically detects GJB1 in WB, IF, FACS and IHC (fro). It exhibits reactivity toward Rat and Mouse.

Quick Overview for GJB1 antibody (CF®488A) (ABIN6178500)

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Reactivity: Rat, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This GJB1 antibody is conjugated to CF®488A

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: R5-21C

Product Details anti-GJB1 Antibody (CF®488A)

Purpose: Rat Monoclonal anti-Connexin-32 (R5.21C), CF488A Conjugate

Characteristics: This antibody recognizes a protein of 27-32 kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane ?-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities, therefore, one connexin usually cannot fully substitute for another. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Mouse liver DOC-JR-plasma membranes

Isotype: IgG2a

Application Details

Application Notes: Immunohistology (frozen) 0.5-1 μg/mL

• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells
• Optimal dilution for a specific application should be determined by user

Comment:

MCF-7 Cells. Pancreas, Liver, Kidney, Stomach or Tonsil

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for GJB1

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Alternative Name: Connexin-32

Background: Charcot Marie Tooth neuropathy X linked, CMTX, CMTX1, Connexin-32, Cx32, GAP junction 28  kDa liver protein, Gap junction beta-1 protein, Gap junction protein beta 1 32kD, GJB1

Molecular Weight: 27-32 kDa

Gene ID: 14618

Pathways: Cell-Cell Junction Organization