Serotonin Receptor 5A antibody (AA 17-34)

Catalog No. ABIN617921

The Rabbit Polyclonal anti-Serotonin Receptor 5A antibody has been validated for WB, IF, IHC, ICC and IEM. It is suitable to detect Serotonin Receptor 5A in samples from Rat and Clams (Mytilus). There are 2+ publications available.

Quick Overview for Serotonin Receptor 5A antibody (AA 17-34) (ABIN617921)

Target: Serotonin Receptor 5A (HTR5A)

Reactivity: Rat, Clams (Mytilus)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Serotonin Receptor 5A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoelectron Microscopy (IEM)

Product Details anti-Serotonin Receptor 5A Antibody

Binding Specificity: AA 17-34

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Characteristics: The 5-HT 5A Receptor Antibody was raised against synthetic peptide sequence corresponding to amino acids 17-34 of the rat 5-HT5A receptor coupled to carrier protein with glutaraldehyde. The 5-HT5A Receptor was quality control tested using standard immunohisto-chemical methods. The antiserum demonstrates strongly positive labeling of rat cortex and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/100 - 1/300 in PBS/0.3 % Triton X-100 - Cy3 and 1/200 - 1/400 in PBS/0.3 % Triton X-100 - Bn/Av-HRP. Intensification methods such as nickel will approximately double the dilution factor as recommended. The antibody was characterized by immunoblotting and immunohistochemistry. Immunoblots of rat brain extracts revealed the presence of two bands at molecular weights of 41 and 47 kD. The lower weight band agrees with the calculated molecular weight based on amino acid sequence. The higher weight may represent glycosylated receptor protein. Immunohistochemical staining of rat brain correlates well with Northern blot analysis and in situ hybridization studies. Immunolabeling is completely abolished by preadsorption with synthetic rat 5-HT5A receptor (17-34). BlastP database sequence homology searches indicate that the amino acid sequence is unique to rat and mouse 5-HT5A receptor.

Purification: Affinity Purified

Immunogen: Rat 5-HT5A receptor (17-34)

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Contains ≤ 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: After reconstitution, use immediately or refrigerate at 2 - 8 °C up to 2 days. For long-term storage aliquot antibody and freeze at -15 °C or lower. Avoid repeated freeze/thaw cycles.

References for anti-Serotonin Receptor 5A antibody (ABIN617921)

Kreiling, Stephens, Reinisch: "A mixture of environmental contaminants increases cAMP-dependent protein kinase in Spisula embryos." in: Environmental toxicology and pharmacology, Vol. 19, Issue 1, pp. 9-18, (2011) (PubMed).

Doly, Fischer, Brisorgueil, Vergé, Conrath: "5-HT5A receptor localization in the rat spinal cord suggests a role in nociception and control of pelvic floor musculature." in: The Journal of comparative neurology, Vol. 476, Issue 4, pp. 316-29, (2004) (PubMed).

Target Details for Serotonin Receptor 5A

Target: Serotonin Receptor 5A (HTR5A)

Background: Other Names:
5-hydroxytryptamine receptor 5A, 5-HT5A, REC17,5ht5a,MR22,5-hydroxytryptamine (serotonin) receptor 5A, G protein-coupled

Gene ID: 25689

Pathways: JAK-STAT Signaling