Serotonin Receptor 1A antibody (AA 294-312)

Catalog No. ABIN617930

The Rabbit Polyclonal anti-Serotonin Receptor 1A antibody has been validated for IHC, IF, ICC and IEM. It is suitable to detect Serotonin Receptor 1A in samples from Human, Rat, Mouse, Monkey and Guinea Pig. There are 5+ publications available.

Quick Overview for Serotonin Receptor 1A antibody (AA 294-312) (ABIN617930)

Target: Serotonin Receptor 1A (HTR1A)

Reactivity: Human, Rat, Mouse, Monkey, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Serotonin Receptor 1A antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoelectron Microscopy (IEM)

Product Details anti-Serotonin Receptor 1A Antibody

Binding Specificity: AA 294-312

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Characteristics: The 5-HT 1A Receptor Antibody was raised against synthetic peptide sequence corresponding to amino acids 294-312 of the rat 5-HT1A receptor. The antiserum is provided as 100 μL of affinity purified serum containing 1 % BSA. The antiserum demonstrates strongly positive labeling of rat cortex, arcuate and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/100 - 1/200 in PBS - Bn/Av-HRP detection. Intensification methods such as nickel will approximately double the dilution factor as recommended. The antibody was characterized by immunohistochemistry and Western blot. Western blot showed a single band of approximately 45 kD. Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography. BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human 5-HT1A receptors.

Purification: Affinity Purified

Immunogen: Rat 5-HT1A receptor (294-312)

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Contains ≤ 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: After reconstitution, use immediately or refrigerate at 2 - 8 °C up to 2 days. For long-term storage aliquot antibody and freeze at -15 °C or lower. Avoid repeated freeze/thaw cycles.

References for anti-Serotonin Receptor 1A antibody (ABIN617930)

Lee, Ahn, Won: "New expression of 5-HT1A receptor in astrocytes in the gerbil hippocampal CA1 region following transient global cerebral ischemia." in: Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology, Vol. 36, Issue 3, pp. 383-9, (2016) (PubMed).

Wang, Wang, Zou, Qu, Liu, Fei, Xia, Needleman, Mikami, Wood: "Mast cell expression of the serotonin1A receptor in guinea pig and human intestine." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 304, Issue 10, pp. G855-63, (2013) (PubMed).

Ahn, Balaban: "Distribution of 5-HT1B and 5-HT1D receptors in the inner ear." in: Brain research, Vol. 1346, pp. 92-101, (2010) (PubMed).

Hadjighassem, Austin, Szewczyk, Daigle, Stockmeier, Albert: "Human Freud-2/CC2D1B: a novel repressor of postsynaptic serotonin-1A receptor expression." in: Biological psychiatry, Vol. 66, Issue 3, pp. 214-22, (2009) (PubMed).

Chalazonitis, Pham, Li, Roman, Guha, Gomes, Kan, Kessler, Gershon: "Bone morphogenetic protein regulation of enteric neuronal phenotypic diversity: relationship to timing of cell cycle exit." in: The Journal of comparative neurology, Vol. 509, Issue 5, pp. 474-92, (2008) (PubMed).

Target Details for Serotonin Receptor 1A

Target: Serotonin Receptor 1A (HTR1A)

Background: Other Names:
5-hydroxytryptamine receptor 1A, ADRB2RL1, ADRBRL1,RAT5HT1A, 5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled

Gene ID: 24473

Pathways: JAK-STAT Signaling, Synaptic Membrane, Feeding Behaviour