DC-SIGN/CD209 antibody (CF®647)

Catalog No. ABIN6179474

This anti-DC-SIGN/CD209 antibody is a Mouse Monoclonal antibody detecting DC-SIGN/CD209 in FACS, IHC, IF and ISt. Suitable for Human.

Quick Overview for DC-SIGN/CD209 antibody (CF®647) (ABIN6179474)

Target: DC-SIGN/CD209 (CD209) (CD209)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DC-SIGN/CD209 antibody is conjugated to CF®647

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunostaining (ISt)

Clone: C209-1781

Product Details anti-DC-SIGN/CD209 Antibody (CF®647)

Purpose: Mouse Monoclonal anti-CD209 / DC-SIGN (C209/1781), CF647 Conjugate

Characteristics: DC-SIGN is a transmembrane receptor that is expressed on the surface of dendritic cells and macrophages. It is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization, which allows the receptor to bind multivalent ligands with high avidity. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human CD209 protein fragment

Isotype: IgG2b kappa

Application Details

Application Notes: Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA Buffer  pH 9.0for 10-20 min followed by cooling at RT for 20 min

• Immunohistology (formalin) 0.5 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

THP-1 or HeLa Cells. Small Intestine, Uterus or Lymph Node.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for DC-SIGN/CD209

Target: DC-SIGN/CD209 (CD209) (CD209)

Alternative Name: CD209 / DC-SIGN

Background: CD209, CDSIGN, CIRE, CLEC4L, DC-SIGN, DC-SIGN1, DCSIGN, Dendritic cell-specific ICAM-3 Grabbing Non-integrin 1, HIV GP120 Binding Protein, SIGN-R1, SIGNR5

Molecular Weight: 45 kDa

Gene ID: 30835, 278694

UniProt: Q9NNX6