FSHR antibody (CF®647)

Catalog No. ABIN6179512

The Mouse Monoclonal anti-FSHR antibody has been validated for IHC, IF and FACS. It is suitable to detect FSHR in samples from Human.

Quick Overview for FSHR antibody (CF®647) (ABIN6179512)

Target: FSHR (Follicle Stimulating Hormone Receptor (FSHR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FSHR antibody is conjugated to CF®647

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: FSHR-1400

Product Details anti-FSHR Antibody (CF®647)

Purpose: Mouse Monoclonal anti-FSH Receptor (FSHR/1400), CF647 Conjugate

Characteristics: Follicle-stimulating hormone receptor (FSHR) is a 695 amino acid G protein coupled receptor. FSH binds to the receptor in a hand-clasp fashion via its ? and ? subunits. While the ? subunit of FSH is involved in the binding of FSH to the receptor, the ? subunit stabilizes this interaction. Linkage studies suggest that a missense mutation in the FSHR gene can cause reduced FSH binding affinity and lead to a condition known as hypergonadotropic ovarian dysgenesis (ODG). In males however, this mutation does not appear to have a detrimental affect on fertility. It is believed that a mutation in the FSHR gene is also associated with ovarian hyperstimulation syndrome, a condition characterized by the presence of multiple serous and hemorrhagic follicular cysts lined by luteinized cells. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length FSHR protein

Isotype: IgG1

Application Details

Application Notes: Immunohistology (formalin) 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA
•   pH 9.0
• or 10 mM citrate buffer
•   pH 6.0
• for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

Uterine Carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for FSHR

Target: FSHR (Follicle Stimulating Hormone Receptor (FSHR))

Alternative Name: FSH Receptor

Background: Follicle-stimulating hormone receptor, Follitropin receptor, FSH receptor, FSH-R, FSHRO, LGR1, ODG1, ovarian dysgenesis 1

Molecular Weight: 75 kDa

Gene ID: 2492, 1428

UniProt: P23945

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Platelet-derived growth Factor Receptor Signaling