GRP94 antibody (CF®568)

Catalog No. ABIN6179632

The Rat Monoclonal anti-GRP94 antibody has been validated for WB, FACS, IF and IHC (f). It is suitable to detect GRP94 in samples from Human, Mouse, Rat, Cow, Chicken, Hamster, Dog, Rabbit, Pig, Guinea Pig, Monkey, Sheep, Horse and Xenopus laevis.

Quick Overview for GRP94 antibody (CF®568) (ABIN6179632)

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reactivity: Human, Mouse, Rat, Cow, Chicken, Hamster, Dog, Rabbit, Pig, Guinea Pig, Monkey, Sheep, Horse, Xenopus laevis

Host: Rat

Clonality: Monoclonal

Conjugate: This GRP94 antibody is conjugated to CF®568

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 9G10-F8-2

Product Details anti-GRP94 Antibody (CF®568)

Purpose: Rat Monoclonal anti-Glucose Regulated Protein 94 (GRP94) (9G10.F8.2), CF568 Conjugate

Characteristics: This antibody recognizes a protein of 94 kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp s are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified glucose regulated protein 94 (grp94) from chicken oviducts

Isotype: IgG2a kappa

Application Details

Application Notes: Immunohistology (Formalin-fixed) 0.5-1.0 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 0.5-1.0 μg/mL
• Western blotting 0.5-1.0 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

U20S cells. About 50% of breast tumors express grp94 protein

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for GRP94

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Alternative Name: Glucose Regulated Protein 94

Molecular Weight: 94 kDa

Gene ID: 7184, 192374

UniProt: P14625

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades