MITF antibody (Biotin)

Catalog No. ABIN6179951

This anti-MITF antibody is a Mouse Monoclonal antibody detecting MITF in IHC, IF and FACS. Suitable for Human and Dog.

Quick Overview for MITF antibody (Biotin) (ABIN6179951)

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reactivity: Human, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This MITF antibody is conjugated to Biotin

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: MITF-915

Product Details anti-MITF Antibody (Biotin)

Purpose: Mouse Monoclonal anti-MiTF (Microphthalmia Transcription Factor) (MITF/915), Biotin Conjugate

Characteristics: MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. This MAb recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human MiTF protein

Isotype: IgG1 kappa

Application Details

Application Notes: Does not react with mouse or rat, others not tested

• Immunohistology (formalin)
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

Jurkat, A-431, HeLa or 501 Mel human melanoma cells or Melanoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Alternative Name: MiTF (Microphthalmia Transcription Factor)

Background: BHLHE32, Class E basic helix-loop-helix protein 32 (bHLHe32), CMM8, Mi, Microphthalmia-associated transcription factor, MITF, WS2, WS2A

Molecular Weight: 52-56 kDa (doublet)

Gene ID: 4286

UniProt: O75030

Pathways: Chromatin Binding