PODXL antibody (Extracellular Domain) (CF®405S)

Catalog No. ABIN6180264

This Mouse Monoclonal antibody specifically detects PODXL in WB, IP, FACS, IF and IHC (f). It exhibits reactivity toward Human, Rat and Rabbit.

Quick Overview for PODXL antibody (Extracellular Domain) (CF®405S) (ABIN6180264)

Target: PODXL (Podocalyxin-Like (PODXL))

Reactivity: Human, Rat, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This PODXL antibody is conjugated to CF®405S

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 3D3

Product Details anti-PODXL Antibody (CF®405S)

Binding Specificity: Extracellular Domain

Purpose: Mouse Monoclonal anti-Podocalyxin (3D3), CF405S Conjugate

Characteristics: Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human podocalyxin

Isotype: IgG1

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Immunoprecipitation 1-2 μg/500 μg protein
• Optimal dilution for a specific application should be determined by user

Comment:

HeLa, Raji, Jurkat cells, Angiosarcoma, Breast, Prostate, Liver, Pancreatic, Kidney

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for PODXL

Target: PODXL (Podocalyxin-Like (PODXL))

Alternative Name: Podocalyxin

Background: PODXL, PDXL, GCTM-2, Gp200, PCLP1, Pcx, Podocalyxin-like protein 1

Molecular Weight: 165-170 kDa

Gene ID: 5420, 732423

UniProt: O00592

Pathways: Tube Formation