PODXL antibody (Extracellular Domain) (CF®568)

Catalog No. ABIN6180278

This anti-PODXL antibody is a Mouse Monoclonal antibody detecting PODXL in WB, IP, FACS, IF and IHC (f). Suitable for Human, Rat and Rabbit.

Quick Overview for PODXL antibody (Extracellular Domain) (CF®568) (ABIN6180278)

Target: PODXL (Podocalyxin-Like (PODXL))

Reactivity: Human, Rat, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This PODXL antibody is conjugated to CF®568

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 3D3

Product Details anti-PODXL Antibody (CF®568)

Binding Specificity: Extracellular Domain

Purpose: Mouse Monoclonal anti-Podocalyxin (3D3), CF568 Conjugate

Characteristics: Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human podocalyxin

Isotype: IgG1

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Immunoprecipitation 1-2 μg/500 μg protein
• Optimal dilution for a specific application should be determined by user

Comment:

HeLa, Raji, Jurkat cells, Angiosarcoma, Breast, Prostate, Liver, Pancreatic, Kidney

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for PODXL

Target: PODXL (Podocalyxin-Like (PODXL))

Alternative Name: Podocalyxin

Background: PODXL, PDXL, GCTM-2, Gp200, PCLP1, Pcx, Podocalyxin-like protein 1

Molecular Weight: 165-170 kDa

Gene ID: 5420, 732423

UniProt: O00592

Pathways: Tube Formation