GFAP antibody (CF®594)

Catalog No. ABIN6180668

The CF®594-conjugated Mouse Monoclonal anti-GFAP antibody (Clone GA-5) (ABIN6180668) specifically detects GFAP in WB, IF, FACS and IHC (f). The antibody is reactive with Human, Rat, Mouse, Cow, Pig, Chicken and Rabbit samples.

Quick Overview for GFAP antibody (CF®594) (ABIN6180668)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Human, Rat, Mouse, Cow, Pig, Chicken, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFAP antibody is conjugated to CF®594

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: GA-5

Product Details anti-GFAP Antibody (CF®594)

Purpose: Mouse Monoclonal anti-GFAP (GA-5), CF594 Conjugate

Characteristics: This MAb recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: GFAP isolated from pig spinal cord

Isotype: IgG1

Application Details

Application Notes: Immunohistology formalin-fixed 0.25-0.5 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

Brain or Astrocytoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP

Background: Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)

Molecular Weight: ~50 kDa

Gene ID: 2670, 514227

UniProt: P14136