Recombinant Cadherin-16 antibody (CF®405S)

Catalog No. ABIN6182962

The Rabbit Monoclonal anti-Cadherin-16 antibody has been validated for IHC, IF and FACS. It is suitable to detect Cadherin-16 in samples from Human, Mouse and Rat.

Quick Overview for Recombinant Cadherin-16 antibody (CF®405S) (ABIN6182962)

Target: Cadherin-16 (CDH16)

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Cadherin-16 antibody is conjugated to CF®405S

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: CDH16-1532R

Product Details anti-Cadherin-16 Antibody (CF®405S)

Purpose: Recombinant Rabbit Monoclonal anti-Ksp-Cadherin / CDH16 (CDH16/1532R), CF405S Conjugate

Characteristics: This MAb recognizes a protein of 130 kDa, identified as Ksp-cadherin. Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels. Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length CDH16 protein

Isotype: IgG kappa

Application Details

Application Notes: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA Buffer  pH 9.0for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

Normal kidney or renal cell carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Cadherin-16

Target: Cadherin-16 (CDH16)

Alternative Name: Ksp-Cadherin / CDH16

Background: Cadherin-16 (CDH16), Kidney-specific cadherin, Ksp-cadherin antibody

Molecular Weight: 130 kDa

Gene ID: 1014, 513660

UniProt: O75309