CD147 antibody (CF®640R)

Catalog No. ABIN6183232

The Mouse Monoclonal anti-CD147 antibody has been validated for FACS, IF, IHC (f) and AGG. It is suitable to detect CD147 in samples from Human.

Quick Overview for CD147 antibody (CF®640R) (ABIN6183232)

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD147 antibody is conjugated to CF®640R

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Aggregation (AGG)

Clone: 8D6

Product Details anti-CD147 Antibody (CF®640R)

Purpose: Mouse Monoclonal anti-CD147 (8D6), CF640R Conjugate

Characteristics: This MAb recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147. It is expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. It stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human T cell line Molt 13

Isotype: IgG1

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1:100-1:200
• Inhibits homotypic aggregation, adhesion to matrix, and migation thro ugh matrigel, order Ab without BSA/azide
• Optimal dilution for a specific application should be determined by user

Comment:

HSB2 cells. Renal Cell, Ovarian carcinoma or Melanoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for CD147

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Alternative Name: CD147

Molecular Weight: 35 kDa (non-reduced), 40 kDa (reduced)

Gene ID: 682, 501293

UniProt: P35613

Pathways: S100 Proteins