Recombinant CD235a/GYPA antibody (CF®594)

Catalog No. ABIN6184772

This anti-CD235a/GYPA antibody is a Rabbit Monoclonal antibody detecting CD235a/GYPA in FACS, IHC and IF. Suitable for Human.

Quick Overview for Recombinant CD235a/GYPA antibody (CF®594) (ABIN6184772)

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD235a/GYPA antibody is conjugated to CF®594

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: GYPA-1725R

Product Details anti-CD235a/GYPA Antibody (CF®594)

Purpose: Recombinant Rabbit Monoclonal anti-Glycophorin-A / CD235a (GYPA/1725R), CF594 Conjugate

Characteristics: This antibody recognizes a sialoglycoprotein of 39 kDa, identified as glycophorin A (GPA). It is present on red blood cells (RBC) and erythroid precursor cells. It has been shown that glycophorin acts as the receptor for Sandei virus and parvovirus. Glycophorins A (GPA) and B (GPB), which are single, trans-membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length Glycophorin A protein

Isotype: IgG kappa

Application Details

Application Notes: Immunohistology (formalin) 0.25-0.5 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

Erythrocytes in any human tissues

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for CD235a/GYPA

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Alternative Name: Glycophorin-A / CD235a

Background: Blood group--MN locus, GPA, GPErik, GpMiIII, GPSAT, GYPA, MN sialoglycoprotein, MNS, PAS2, Sialoglycoprotein alpha

Molecular Weight: 39 kDa

Gene ID: 2993, 2994

UniProt: P02724

Pathways: Maintenance of Protein Location