Transferrin Receptor antibody (CF®647)

Catalog No. ABIN6185212

The Mouse Monoclonal anti-Transferrin Receptor antibody has been validated for FACS, WB and IF. It is suitable to detect Transferrin Receptor in samples from Human.

Quick Overview for Transferrin Receptor antibody (CF®647) (ABIN6185212)

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transferrin Receptor antibody is conjugated to CF®647

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF)

Clone: DF1513

Product Details anti-Transferrin Receptor Antibody (CF®647)

Purpose: Mouse Monoclonal anti-CD71 (DF1513), CF647 Conjugate

Characteristics: This antibody recognizes a ~90-95 kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190 kDa (Workshop IV). This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: KG1 acute myeloid leukemia cell line

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry 0.5-1 μg/million cells/0.1 mL

• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1.0 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

CCRF-CEM, Jurkat or K-562. Human placenta or breast carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Alternative Name: CD71

Background: Mtvr-1, p90, TFR1, TFRC transferrin receptor (p90 CD71), TRFR

Molecular Weight: 85-95 kDa (monomer), 190 kDa (dimer)

Gene ID: 7037, 529618

UniProt: P02786

Pathways: Transition Metal Ion Homeostasis