Transferrin Receptor antibody (AA 94-212) (CF®405S)

Catalog No. ABIN6185318

This anti-Transferrin Receptor antibody is a Mouse Monoclonal antibody detecting Transferrin Receptor in FACS, WB, IHC, ELISA, IF and Coat. Suitable for Human.

Quick Overview for Transferrin Receptor antibody (AA 94-212) (CF®405S) (ABIN6185318)

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transferrin Receptor antibody is conjugated to CF®405S

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Coating (Coat)

Clone: TFRC-1817

Product Details anti-Transferrin Receptor Antibody (CF®405S)

Binding Specificity: AA 94-212

Purpose: Mouse Monoclonal anti-CD71 / Transferrin Receptor (TFRC/1817), CF405S Conjugate

Characteristics: This antibody recognizes a ~90-95 kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190 kDa. This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human TFRC protein fragment (aa94-212) (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes: Flow cytometry 0.5-1 μg/million cells in 0.1 mL

• Immunohistology (formalin) 1-2 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
• ELISA 2-4 μg/mL for coating (order Ab without BSA)
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

PC3 cells. Human placenta or breast carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Alternative Name: CD71 / Transferrin Receptor

Background: Mtvr-1, p90, TFR1, TFRC transferrin receptor (p90 CD71), TRFR

Molecular Weight: 85-95 kDa (monomer), 190 kDa (dimer)

Gene ID: 7037, 529618

UniProt: P02786

Pathways: Transition Metal Ion Homeostasis