Transferrin Receptor antibody (AA 94-212) (CF®568)

Catalog No. ABIN6185332

The Mouse Monoclonal anti-Transferrin Receptor antibody has been validated for FACS, WB, IHC, ELISA, IF and Coat. It is suitable to detect Transferrin Receptor in samples from Human.

Quick Overview for Transferrin Receptor antibody (AA 94-212) (CF®568) (ABIN6185332)

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transferrin Receptor antibody is conjugated to CF®568

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Coating (Coat)

Clone: TFRC-1817

Product Details anti-Transferrin Receptor Antibody (CF®568)

Binding Specificity: AA 94-212

Purpose: Mouse Monoclonal anti-CD71 / Transferrin Receptor (TFRC/1817), CF568 Conjugate

Characteristics: This antibody recognizes a ~90-95 kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190 kDa. This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human TFRC protein fragment (aa94-212) (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes: Flow cytometry 0.5-1 μg/million cells in 0.1 mL

• Immunohistology (formalin) 1-2 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
• ELISA 2-4 μg/mL for coating (order Ab without BSA)
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

PC3 cells. Human placenta or breast carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Alternative Name: CD71 / Transferrin Receptor

Background: Mtvr-1, p90, TFR1, TFRC transferrin receptor (p90 CD71), TRFR

Molecular Weight: 85-95 kDa (monomer), 190 kDa (dimer)

Gene ID: 7037, 529618

UniProt: P02786

Pathways: Transition Metal Ion Homeostasis