PIP antibody (AA 41-146) (CF®647)

Catalog No. ABIN6186048

This Mouse Monoclonal antibody specifically detects PIP in WB, IHC, IF, FACS and ISt. It exhibits reactivity toward Human.

Quick Overview for PIP antibody (AA 41-146) (CF®647) (ABIN6186048)

Target: PIP (Prolactin-Induced Protein (PIP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PIP antibody is conjugated to CF®647

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt)

Clone: PIP-1571

Product Details anti-PIP Antibody (CF®647)

Binding Specificity: AA 41-146

Purpose: Mouse Monoclonal anti-GCDFP-15 (PIP/1571), CF647 Conjugate

Characteristics: This antibody recognizes a protein of 15 kDa, identified as Gross cystic disease fluid protein 15 (GCDFP-15). It is a major protein component of benign breast gross cysts. It is a known marker of breast cancer, as it is found in approximately 50 % of all breast cancer specimens. GCDFP-15, also known as PIP, for prolactin inducible protein, is a prolactin and androgen controlled protein. This antibody is useful in the identification of metastatic breast carcinoma, or fluid analysis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human GCDFP-15 protein fragment (aa 41-146) (exact sequence is proprietary)

Isotype: IgG2a kappa

Application Details

Application Notes: Immunohistology (formalin) 0.5-1 μg/mL

•   pH 9.0
• for 10-20 min followed by cooling at RT for 20 min
• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris buffer with1 mM EDTA
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HepG2 Cells. Breast or Pancreas.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for PIP

Target: PIP (Prolactin-Induced Protein (PIP))

Alternative Name: GCDFP-15

Background: BRST-2, GCDFP-15, gp17, GPIP4, Gross cystic disease fluid protein 15, Prolactin-induced protein (PIP), Prolactin-inducible protein (PIP), Secretory actin-binding protein (SABP)

Molecular Weight: 15 kDa

Gene ID: 5304, 99949

UniProt: P12273