Villin 1 antibody (AA 179-311) (CF®647)

Catalog No. ABIN6186922

The Mouse Monoclonal anti-Villin 1 antibody has been validated for IHC, WB, IF and FACS. It is suitable to detect Villin 1 in samples from Human.

Quick Overview for Villin 1 antibody (AA 179-311) (CF®647) (ABIN6186922)

Target: Villin 1 (VIL1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Villin 1 antibody is conjugated to CF®647

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: VIL1-1314

Product Details anti-Villin 1 Antibody (CF®647)

Binding Specificity: AA 179-311

Purpose: Mouse Monoclonal anti-Villin (VIL1/1314), CF647 Conjugate

Characteristics: This antibody recognizes a protein of 95 kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Villin fragment of 133 amino acid residues (aa179-311) (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry 0.5-1 μg/million cells in 0.1 mL

• Immunohistology (formalin) 0.25-0.5 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Western blotting 1-2 μg/mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

A549, HepG2 and HCT116 cells. Colon or Rectum.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Villin 1

Target: Villin 1 (VIL1)

Alternative Name: Villin

Background: VIL1, Villin-1, Villin1

Molecular Weight: 93 kDa

Gene ID: 7429, 654595

UniProt: P09327

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization