Villin 1 antibody (AA 179-311)
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- Target See all Villin 1 (VIL1) Antibodies
- Villin 1 (VIL1)
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Binding Specificity
- AA 179-311
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Villin 1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)
- Specificity
- Recognizes a protein of 95 kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
- Purification
- Purified by Protein A/G
- Immunogen
- Recombinant human Villin fragment (around aa179-311) (exact sequence is proprietary)
- Clone
- VIL1-1314
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product VIL1 Primary Antibody
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anti-Villin 1 (VIL1) (AA 1-320) antibody
VIL1 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Villin 1 (VIL1) (AA 180-207), (N-Term) antibodyVIL1 Reactivity: Human, Mouse WB, FACS, IHC (p) Host: Rabbit Polyclonal RB17824 unconjugated
anti-Villin 1 (VIL1) (AA 1-209) antibodyVIL1 Reactivity: Human WB, IHC, FACS, ELISA, ICC Host: Mouse Monoclonal 3E5G11 unconjugated
anti-Villin 1 (VIL1) (N-Term) antibodyVIL1 Reactivity: Human WB, IF, IHC (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Villin 1 (VIL1) (AA 1-209) antibodyVIL1 Reactivity: Human WB, IHC, ELISA Host: Mouse Monoclonal 5E3B2 unconjugated
anti-Villin 1 (VIL1) (AA 1-421) antibodyVIL1 Reactivity: Human WB, IP Host: Rabbit Polyclonal unconjugated
anti-Villin 1 (VIL1) (AA 179-311) antibodyVIL1 Reactivity: Human IHC, StM Host: Mouse Monoclonal rVIL1-1325 unconjugated Recombinant Antibody
anti-Villin 1 (VIL1) (AA 1-421) antibodyVIL1 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 2F10 unconjugated
anti-Villin 1 (VIL1) (AA 600-700) antibodyVIL1 Reactivity: Human IHC Host: Rabbit Monoclonal MSVA-459R unconjugated Recombinant Antibody
anti-Villin 1 (VIL1) (AA 770-799), (C-Term) antibodyVIL1 Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Positive Control: A549, HepG2 and HCT116 cells. Colon or Rectum.
Known Application: Western Blot (1-2 μg/mL for 60 minutes at RT), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- Villin 1 (VIL1)
- Alternative Name
- VIL1 (VIL1 Products)
- Molecular Weight
- 93kDa
- Gene ID
- 7429
- UniProt
- P09327
- Pathways
- EGFR Signaling Pathway, Regulation of Actin Filament Polymerization
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