CD63 antibody
Quick Overview for CD63 antibody (ABIN6188015)
Target
See all CD63 AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
-
-
Purpose
- Mouse Monoclonal anti-CD63 (MX-49.129.5), Purified, BSA-free
-
Characteristics
- This MAb recognizes protein of 26 kDa-60 kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
-
Purification
- Purified
-
Immunogen
- Full length CD63 of human origin
-
Isotype
- IgG1 kappa
-
-
-
-
Application Notes
-
Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
-
Comment
-
SK-MEL-28, HL60, THP-1 or NIH/3T3 cells. Melanoma or lymphoma.
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Concentration
- 1 mg/mL
-
Buffer
- PBS (no BSA, no azide)
-
Preservative
- Azide free
-
-
- CD63
-
Alternative Name
- CD63
-
Molecular Weight
- 26 kDa (core protein), 30-60 kDa (glycosylated)
-
Gene ID
- 967, 445570
-
UniProt
- P08962
Target
-