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CD63 antibody

This anti-CD63 antibody is a Mouse Monoclonal antibody detecting CD63 in FACS, WB, IF, ISt and IHC (f). Suitable for Human and Mouse.
Catalog No. ABIN6188015

Quick Overview for CD63 antibody (ABIN6188015)

Target

See all CD63 Antibodies
CD63

Reactivity

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Human, Mouse

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This CD63 antibody is un-conjugated

Application

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Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone

MX-49-129-5
  • Purpose

    Mouse Monoclonal anti-CD63 (MX-49.129.5), Purified, BSA-free

    Characteristics

    This MAb recognizes protein of 26 kDa-60 kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    Purification

    Purified

    Immunogen

    Full length CD63 of human origin

    Isotype

    IgG1 kappa
  • Application Notes

    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Western blotting 0.5-1 μg/mL
    • Optimal dilution for a specific application should be determined by user

    Comment

    SK-MEL-28, HL60, THP-1 or NIH/3T3 cells. Melanoma or lymphoma.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    PBS (no BSA, no azide)

    Preservative

    Azide free
  • Target

    CD63

    Alternative Name

    CD63

    Molecular Weight

    26 kDa (core protein), 30-60 kDa (glycosylated)

    Gene ID

    967, 445570

    UniProt

    P08962
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