ODC1 antibody (CF®594)

Catalog No. ABIN6188914

The Mouse Monoclonal anti-ODC1 antibody has been validated for WB, IF, FACS and IHC (f). It is suitable to detect ODC1 in samples from Human and Rat.

Quick Overview for ODC1 antibody (CF®594) (ABIN6188914)

Target: ODC1 (Ornithine Decarboxylase 1 (ODC1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ODC1 antibody is conjugated to CF®594

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: ODC1-486

Product Details anti-ODC1 Antibody (CF®594)

Purpose: Mouse Monoclonal anti-ODC-1 (Ornithine Decarboxylase 1) (ODC1/486), CF594 Conjugate

Characteristics: This antibody recognizes a 53 kDa protein, identified as the Ornithine Decarboxylase (ODC-1). ODC is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. ODC activity in colorectal carcinomas is greater than those in adenomas and normal mucosa. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human ODC-1 protein

Isotype: IgG2a kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.25-0.5 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

PC3 cells. Placenta, Prostate or Testicular carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for ODC1

Target: ODC1 (Ornithine Decarboxylase 1 (ODC1))

Alternative Name: ODC-1 (Ornithine Decarboxylase 1)

Background: Ornithine decarboxylase structural 1, RNODC

Molecular Weight: 53 kDa

Gene ID: 4953, 467701

UniProt: P11926