PAX7 antibody (AA 300-600) (CF®488A)

Catalog No. ABIN6189140

This anti-PAX7 antibody is a Mouse Monoclonal antibody detecting PAX7 in IHC (f). Suitable for Human.

Quick Overview for PAX7 antibody (AA 300-600) (CF®488A) (ABIN6189140)

Target: PAX7 (Paired Box 7 (PAX7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAX7 antibody is conjugated to CF®488A

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: PAX7-1187

Product Details anti-PAX7 Antibody (CF®488A)

Binding Specificity: AA 300-600

Purpose: Mouse Monoclonal anti-PAX7 (PAX7/1187), CF488A Conjugate

Characteristics: The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma). Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (aa300-600) of human PAX7 protein

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Optimal dilution for a specific application should be determined by user

Comment:

Rhabdomyosarcoma (RMS) or Testicular Carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for PAX7

Target: PAX7 (Paired Box 7 (PAX7))

Alternative Name: PAX7

Background: HUP1, Paired box gene 7, Paired box homeotic gene 7, Paired domain gene HuP1, PAX7 transcriptional factor, PAX7/FKHR fusion gene, PAX7B, RMS2

Molecular Weight: 57 kDa

Gene ID: 5081, 113253

UniProt: P23759