PAX7 antibody (AA 300-600) (CF®488A)

Catalog No. ABIN6189178

The Mouse Monoclonal anti-PAX7 antibody has been validated for WB, FACS, IF and IHC (fro). It is suitable to detect PAX7 in samples from Human, Mouse, Rat, Zebrafish (Danio rerio) and Chicken.

Quick Overview for PAX7 antibody (AA 300-600) (CF®488A) (ABIN6189178)

Target: PAX7 (Paired Box 7 (PAX7))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAX7 antibody is conjugated to CF®488A

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: PAX7-497

Product Details anti-PAX7 Antibody (CF®488A)

Binding Specificity: AA 300-600

Purpose: Mouse Monoclonal anti-PAX7 (PAX7/497), CF488A Conjugate

Characteristics: The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma). Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (aa300-600) of human PAX7 protein

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology (frozen) 0.5-1 μg/mL

• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HeLa, Jurkat, A431, MCF-7, U2OS or NIH/3T3 cells. Rhabdomyosarcoma (RMS)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for PAX7

Target: PAX7 (Paired Box 7 (PAX7))

Alternative Name: PAX7

Background: HUP1, Paired box gene 7, Paired box homeotic gene 7, Paired domain gene HuP1, PAX7 transcriptional factor, PAX7/FKHR fusion gene, PAX7B, RMS2

Molecular Weight: 57 kDa

Gene ID: 5081, 113253

UniProt: P23759