GRP78 antibody

Catalog No. ABIN618937

This Goat Polyclonal antibody specifically detects GRP78 in WB. It exhibits reactivity toward Arabidopsis thaliana, Spinach and Zea mays.

Quick Overview for GRP78 antibody (ABIN618937)

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivity: Arabidopsis thaliana, Spinach, Zea mays

Host: Goat

Clonality: Polyclonal

Conjugate: This GRP78 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GRP78 Antibody

Predicted Reactivity: dicots including: Nicotiana tabacum, Spinacia oleracea, monocots: Hordeum vulgare, Oryza sativa, Zea mays, trees: Picea sitchensis, Populus trichocarpa, moss: Physcomitrella patens

Characteristics: Expected / apparent Molecular Weight of the Antigene: 73.5 / 80 kDa

Purification: serum

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP proteins: BiP1 At5g28540 Q9LKR3, BiP2 At5g42020 F4K007 , BiP3 At5g09080 Q8H1B3

Application Details

Application Notes: Recommended Dilution: 1 : 2000 with standard ECL (WB)

Comment:

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Antibody has a reduced reactivity to monocots in western blot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS pH 7.4, lyophilized

Target Details for GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Alternative Name: BiP

Background: AGI Code: At5g28540, At5g42020
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER). Alternative name: AtBP2

Molecular Weight: expected: 73.5 kDa, apparent: 80 kDa

UniProt: Q39043

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling