NKX2-1 antibody (CF®640R)

Catalog No. ABIN6189654

This Mouse Monoclonal antibody specifically detects NKX2-1 in FACS, IF and IHC (f). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NKX2-1 antibody (CF®640R) (ABIN6189654)

Target: NKX2-1 (NK2 Homeobox 1 (NKX2-1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This NKX2-1 antibody is conjugated to CF®640R

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 8G7G3-1

Product Details anti-NKX2-1 Antibody (CF®640R)

Purpose: Mouse Monoclonal anti-TTF-1 / NKX2.1 (8G7G3/1), CF640R Conjugate

Characteristics: This antibody recognizes a protein of 40 kDa, identified as Thyroid transcription factor-1 (TTF-1). TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates.Loss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF-1 reactivity is also seen in thyroid malignancies. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Rat full length TTF-1 recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Predicted to show broad species reactivity
• Optimal dilution for a specific application should be determined by user

Comment:

MAD109, MLE-15, H441-4, or H345 cells. Normal thyroid or lung.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for NKX2-1

Target: NKX2-1 (NK2 Homeobox 1 (NKX2-1))

Alternative Name: TTF-1 / NKX2.1

Molecular Weight: 40 kDa

Gene ID: 7080, 94367

UniProt: P43699

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Glucan Metabolic Process, Feeding Behaviour