ALDH1A1 antibody (AA 315-434) (Biotin)

Catalog No. ABIN6190135

This anti-ALDH1A1 antibody is a Mouse Monoclonal antibody detecting ALDH1A1 in WB, IHC, IF and FACS. Suitable for Human.

Quick Overview for ALDH1A1 antibody (AA 315-434) (Biotin) (ABIN6190135)

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ALDH1A1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: ALDH1A1-1381

Product Details anti-ALDH1A1 Antibody (Biotin)

Binding Specificity: AA 315-434

Purpose: Mouse Monoclonal anti-ALDH1A1 (ALDH1A1/1381), Biotin Conjugate

Characteristics: ALDH1A1 belongs to the ALDH enzymes, a family of evolutionarily conserved enzymes comprised of 19 isoforms that are localized in the cytoplasm, mitochondria or nucleus. ALDH1A1 is predominantly expressed in the epithelium of testis, brain, eye, liver, kidney, as well as neural and hematopoietic stem cells. Reportedly, high ALDH1A1 expression is found in solitary fibrous tumor (SFT) and hemangiopericytoma (HPC), compared to meningiomas and synovial sarcomas. In combination with CD34, ALDH1A1 may be useful for the differentiation among SFT, HPC, meningioma, and synovial sarcoma. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified recombinant fragment of human ALDH1A1 (aa 315-434) (exact sequence is proprietary)

Isotype: IgG1

Application Details

Application Notes: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HepG2 or A549 cells. Colon or Testicular Carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for ALDH1A1

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Alternative Name: ALDH1A1

Molecular Weight: 56 kDa

Gene ID: 216, 76392

UniProt: P00352

Pathways: Dopaminergic Neurogenesis