FTL antibody (AA 38-165) (CF®488A)

Catalog No. ABIN6191686

This anti-FTL antibody is a Mouse Monoclonal antibody detecting FTL in IHC, IF and FACS. Suitable for Human.

Quick Overview for FTL antibody (AA 38-165) (CF®488A) (ABIN6191686)

Target: FTL (Ferritin, Light Polypeptide (FTL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FTL antibody is conjugated to CF®488A

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: FTL-1389

Product Details anti-FTL Antibody (CF®488A)

Binding Specificity: AA 38-165

Purpose: Mouse Monoclonal anti-Ferritin, Light chain (FTL/1389), CF488A Conjugate

Characteristics: Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human FTL protein fragment (aa 38-165) (exact sequence is proprietary)

Isotype: IgG1

Application Details

Application Notes: Immunohistology (formalin) 0.1-0.2 μg/mL

• Flow cytometry 0.1-0.2 μg/million cells Immunofluorescence 0.1-0.2 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Optimal dilution for a specific application should be determined by user

Comment:

HepG2, HeLa, HL-60 or 293T cells. Pancreas, Liver, Cerebellum or Testis.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for FTL

Target: FTL (Ferritin, Light Polypeptide (FTL))

Alternative Name: Ferritin, Light chain

Background: Ferritin L chain, Ferritin L subunit, Ferritin light chain, Ferritin light polypeptide, FTL, LFTD, NBIA3

Molecular Weight: 19-25 kDa

Gene ID: 2512, 433670

UniProt: P02792

Pathways: Transition Metal Ion Homeostasis