ADH/ALDH antibody

Catalog No. ABIN619569

The Rabbit Polyclonal anti-ADH/ALDH antibody (ABIN619569) specifically detects ADH/ALDH in WB. The antibody is reactive with Chlamydomonas reinhardtii, E. coli and Streptococcus pneumoniae samples.

Quick Overview for ADH/ALDH antibody (ABIN619569)

Target: ADH/ALDH

Reactivity: Chlamydomonas reinhardtii, E. coli, Streptococcus pneumoniae

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Product Details

Purpose: Anti-ADH/ALDH | Alcohol/acetaldehyde dehydrogenase (bacterial/algal)

Cross-Reactivity (Details): Chlamydomonas reinhardtii, E.coli, Streptococcus pmenumoniae
Not reactive in: Lactobacillus plantarum

Predicted Reactivity: Algae,Lactococcus lactis,Nannochloropsis gaditana,Picochlorum sp.

Purification: Serum

Immunogen: KLH-conjugated peptide derived from available algal and bacterial ADH sequences including Chlamydomonas reinhardtii

Application Details

Application Notes: 1 : 1000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details

Target: ADH/ALDH

Background:

Background: Alcohol dehydrogenase (E.C.:1.1.1.1) is an important enzyme for plants and microbes. In microalgae and bacteria the conversion of Acetyl-CoA to ethanol under conditions of oxygen deprivation is catalyzed by the dual function enzyme alcohol/acetaldehyde dehydrogenase (ADH/ALDH, E.C.:1.1.1.1 /1.2.1.10). This reaction results in NAD+ recycling and allows glycolysis to proceed.

Additional information: Selected peptide is well conserved in Escherichia coli ADHE (P0A9Q7), most of the microbial dual function aldehyde/alcohol dehydrogenases (ADHE) and Iron-containing alcohol dehydrogenases are also conserved in a peptide used to elicit ADH antibody

Molecular Weight: 100 | 100 kDa (C.reinhardtii), 96 kDa (E.coli)