PSI-C core subunit of photosystem I (PsaC) antibody

Catalog No. ABIN619731

The Rabbit Polyclonal anti-PSI-C core subunit of photosystem I (PsaC) antibody (ABIN619731) specifically detects PSI-C core subunit of photosystem I (PsaC) in WB. The antibody is reactive with Arabidopsis thaliana, Chlamydomonas reinhardtii, Cyanophora paradoxa, Emiliania huxleyi, Euglena gracilis, Gonyaulax polyedra, Heterosigma akashiwo, Hordeum vulgare subsp. spontaneum (Wild barley) (Hordeum spontaneum), Micromonas pusilla, Spinach, Stony Coral (Turbinaria), Synechococcus PCC 7942 and Thalassiosira pseudonana samples.

Quick Overview for PSI-C core subunit of photosystem I (PsaC) antibody (ABIN619731)

Target: PSI-C core subunit of photosystem I (PsaC)

Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Cyanophora paradoxa, Emiliania huxleyi, Euglena gracilis, Gonyaulax polyedra, Heterosigma akashiwo, Hordeum vulgare subsp. spontaneum (Wild barley) (Hordeum spontaneum), Micromonas pusilla, Spinach, Stony Coral (Turbinaria), Synechococcus PCC 7942, Thalassiosira pseudonana

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Product Details

Predicted Reactivity: dicots including Glycine max, Nicotiana tabacum, Spinacia oleracea, and monocots, Physcomitrella patens, algae and cyanobacteria, Prochlorococcus sp. (surface and a deep water ecotype)

Characteristics: Expected / apparent Molecular Weight of the Antigene: 9 kDa

Purification: serum

Immunogen: KLH-conjugated synthetic peptide conserved in all known PsaC proteins includingArabidopsis thaliana AtCg01060, Hordeum vulgare P69416, Oryza sativa P0C360, Chlamydomonas reinhardtii Q00914, Synechococcus elongatus Q31QV2

Application Details

Application Notes: Recommended Dilution: 1 : 1000 with ECL (WB)

Comment:

In some species minor cross reactions with some larger proteins are seen. These may contain related iron-sulfur binding motifs. Therefore size verification of the reacting band is required. Due to the small size of the protein, care should be taken to differentiate between chemiluminescent signal from PsaC and non-specific signals from chlotophylls or lipids if pigment is retained near the bottom of the blot.For the most optimal results use:thylakoid membranes or PSI particles, solubilized in a SDS sample buffer (final concentrations: 63 mM Tris HCl, 10% glycerol, 2% SDS, 0.0025% bromophenol blue) with 2.5% beta-mercaptoethanol at 85C for 2 minutes. The samples were spun softly, then the supernatant loaded.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Target Details

Target: PSI-C core subunit of photosystem I (PsaC)

Background: AGI Code: ATCG01060
PsaC is a conserved, chloroplast-encoded, Fe-S binding protein of approximately 10kDa, present in all known Photosystem I complexes. It is located on the stromal side of the thylacoid membranes. PsaC coordinates the Fe-S clusters FA and FB through two cysteine-rich domains.

Molecular Weight: 9 kDa

UniProt: P69416, Q00914, P0C360, Q31QV2, P62090