DMPO antibody

Catalog No. ABIN619793

The Mouse Monoclonal anti-DMPO antibody is suitable to detect DMPO. It has been validated for IP, WB, ELISA, ICC and IST.

Quick Overview for DMPO antibody (ABIN619793)

Target: DMPO

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunoprecipitation (IP), Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immuno-Spin Trapping (IST)

Clone: N1664A

Product Details

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: DMPO (species independent)

Characteristics: Expected / apparent Molecular Weight of the Antigene: 90 kDa

Purification: affinity purified

Immunogen: 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to ovalbumin

Isotype: IgG1

Application Details

Application Notes: 1-10 μg/mL with standard ECL (WB), 1-10 μg/mL (ELISA), 10 μg/mL (ICC), 25 μg (IP)

Comment:

Important note for immunoprecipitation: do not link antibody to matrix prior to use as this causes large loss of reactivity to DMPO adduct.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage Comment: store at -20°C for one year. Avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details

Target: DMPO

Target Type: Chemical

Background: DMPO (5,5-dimethly-1-proline N-oxide) is a compound used in spin trapping. It is a method employed in chemistry as an analytical technique for detection and identification of short-lived free radicals. DMPO adducts can be generated with protein and DNA radicals.

Molecular Weight: 90 kDa