PTEN antibody (AA 209-403)

Catalog No. ABIN7743840

This anti-PTEN antibody is a Rabbit Polyclonal antibody detecting PTEN in WB, IF and IHC. Suitable for Human. This Primary Antibody has been cited in 2+ publications.

Quick Overview for PTEN antibody (AA 209-403) (ABIN7743840)

Target: PTEN (Phosphatase and Tensin Homolog (PTEN))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTEN antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-PTEN Antibody

Binding Specificity: AA 209-403

Sequence: GTCNPQFVVC QLKVKIYSSN SGPTRREDKF MYFEFPQPLP VCGDIKVEFF HKQNKMLKKD KMFHFWVNTF FIPGPEETSE KVENGSLCDQ EIDSICSIER ADNDKEYLVL TLTKNDLDKA NKDKANRYFS PNFKVKLYFT KTVEEPSNPE ASSSTSVTPD VSDNEPDHYR YSDTTDSDPE NEPFDEDQHT QITKV

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 209-403 of human PTEN (NP_000305.3).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:200

Comment:

HIGH QUALITY

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

References for anti-PTEN antibody (ABIN7743840)

Dinesh, Rasool et al.: "Berberine inhibits IL-21/IL-21R mediated inflammatory proliferation of fibroblast-like synoviocytes through the attenuation of PI3K/Akt signaling pathway and ameliorates IL-21 mediated ..." in: Cytokine, Vol. 106, pp. 54-66, (2018) (PubMed).

Dong, Xu, Yang, Shi, Jiang, Chen, Song, Wang: "Associations of Functional MicroRNA Binding Site Polymorphisms in IL23/Th17 Inflammatory Pathway Genes with Gastric Cancer Risk." in: Mediators of inflammation, Vol. 2017, pp. 6974696, (2018) (PubMed).

Target Details for PTEN

Target: PTEN (Phosphatase and Tensin Homolog (PTEN))

Alternative Name: PTEN

Background: This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.,10q23del,BZS,CWS1,DEC,GLM2,MHAM,MMAC1,PTEN1,TEP1,PTEN,PTENbeta,Epigenetics & Nuclear Signaling,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Cancer,Tumor suppressors,Signal Transduction,Kinase,PI3K-Akt Signaling Pathway,mTOR Signaling Pathway,Cell Biology & Developmental Biology,Apoptosis,Inhibition of Apoptosis,Cell Cycle,Microtubules,Endocrine & Metabolism,Lipid Metabolism,Insulin Receptor Signaling Pathway,Endocrine and metabolic diseases,Diabetes,Metabolic disorders,Obesity,Immunology & Inflammation,Cytokines,B Cell Receptor Signaling Pathway,Cardiovascular,Angiogenesis,Phosphatase of Akt,PTEN

Molecular Weight: 19 kDa/47 kDa/64 kDa

Gene ID: 5728

UniProt: P60484

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Inositol Metabolic Process, Synaptic Membrane, Regulation of Cell Size, Autophagy, Platelet-derived growth Factor Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling