CD3 epsilon antibody (Activation Site) (FITC)

Catalog No. ABIN6240811

This anti-CD3 epsilon antibody is a Mouse Monoclonal antibody detecting CD3 epsilon in ICFC. Suitable for Human and Mouse.

Quick Overview for CD3 epsilon antibody (Activation Site) (FITC) (ABIN6240811)

Target: CD3 epsilon (CD3E)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is conjugated to FITC

Application: Intracellular Flow Cytometry (ICFC)

Clone: APA1-1

Product Details anti-CD3 epsilon Antibody (FITC)

Binding Specificity: Activation Site

Purpose: Anti-CD3 epsilon (activation epitope) FITC

Specificity: The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.

Cross-Reactivity (Details): Human, Mouse

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Purified human CD3 proteins isolated from thymus

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1 μg/mL. Intracellular staining.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3 epsilon

Background: CD3 antigen, epsilon polypeptide,CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.,CD3 epsilon chain, T3E, IMD18

Gene ID: 916

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway